Ascorbic acid and cell survival of adriamycin resistant and sensitive MCF-7 breast tumor cells.
Identifieur interne : 001232 ( Main/Exploration ); précédent : 001231; suivant : 001233Ascorbic acid and cell survival of adriamycin resistant and sensitive MCF-7 breast tumor cells.
Auteurs : W W Wells [États-Unis] ; P A Rocque ; D P Xu ; E B Meyer ; L J Charamella ; N V DimitrovSource :
- Free radical biology & medicine [ 0891-5849 ] ; 1995.
Descripteurs français
- KwdFr :
- Acide ascorbique (MeSH), Animaux (MeSH), Cellules cultivées (effets des médicaments et des substances chimiques), Doxorubicine (pharmacologie), Glutarédoxines (MeSH), Humains (MeSH), Oxidoreductases (MeSH), Protéines (MeSH), Rats (MeSH), Relation dose-effet des médicaments (MeSH), Survie cellulaire (MeSH), Technique de Western (MeSH), Tumeurs du sein (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Cellules cultivées.
- pharmacologie : Doxorubicine.
- Acide ascorbique, Animaux, Glutarédoxines, Humains, Oxidoreductases, Protéines, Rats, Relation dose-effet des médicaments, Survie cellulaire, Technique de Western, Tumeurs du sein.
English descriptors
- KwdEn :
- Animals (MeSH), Ascorbic Acid (MeSH), Blotting, Western (MeSH), Breast Neoplasms (MeSH), Cell Survival (MeSH), Cells, Cultured (drug effects), Dose-Response Relationship, Drug (MeSH), Doxorubicin (pharmacology), Glutaredoxins (MeSH), Humans (MeSH), Oxidoreductases (MeSH), Proteins (MeSH), Rats (MeSH).
- MESH :
- chemical , pharmacology : Doxorubicin.
- chemical : Ascorbic Acid, Glutaredoxins, Oxidoreductases, Proteins.
- drug effects : Cells, Cultured.
- Animals, Blotting, Western, Breast Neoplasms, Cell Survival, Dose-Response Relationship, Drug, Humans, Rats.
Abstract
The ability of human cells to regenerate ascorbic acid from dehydroascorbate is partially dependent on the glutathione redox status of the cell and the relative activity of dehydroascorbate reductases. Mammalian dehydroascorbate reductase activity is associated with two proteins known as thioltransferase (glutaredoxin) and protein disulfide isomerase. We compared the specific activity of thioltransferase, protein disulfide isomerase, and other GSH-related enzymes in Adriamycin-resistant human breast tumor cells, MCF-7 ADRR, and Adriamycin-sensitive, MCF-7 WT, tumor cells. MCF-7 ADRR cells had higher activities of glutathione peroxidase (34.7 fold), nonseleno-glutathione peroxidase (glutathione S-transferases; 5.3 fold), thioredoxin (2.3 fold), and thioltransferase (4.0 fold) compared with the WT Adriamycin-sensitive cell line. Thioltransferase was detected in Western blots in extracts of ADRR MCF-7 cells but not in WT MCF-7 cells. alpha-Tocopherol in the membrane and cytosolic fractions was 2.8 and 3.0 fold higher, respectively, in Adriamycin-resistant compared with Adriamycin-sensitive cells. Supplementation of MCF-7 cells with L-ascorbic acid 2-phosphate (2 and 10 mM) had no effect on WT cell viability after 5 days incubation with up to 0.33 microM Adriamycin. In contrast, supplementation of ADRR MCF-7 cells with L-ascorbic acid 2-phosphate resulted in enhanced resistance up to 3.4 microM Adriamycin over a 5-day incubation. Both lines of MCF-7 cells demonstrated the ability to utilize ascorbic acid as the 2-phosphate derivative. After 48 h incubation with 8.6 microM Adriamycin, the resistant cells maintained normal viability and ascorbate-dehydroascorbate levels, whereas drug-sensitive cells had significantly lower ascorbate with a higher percent dehydroascorbate and increased cell death as judged by cell protein levels (52% of controls).
DOI: 10.1016/0891-5849(94)00188-p
PubMed: 7750794
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ascorbic acid and cell survival of adriamycin resistant and sensitive MCF-7 breast tumor cells.</title><author><name sortKey="Wells, W W" sort="Wells, W W" uniqKey="Wells W" first="W W" last="Wells">W W Wells</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, Michigan State University, East Lansing 48824, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Michigan State University, East Lansing 48824</wicri:regionArea><orgName type="university">Université d'État du Michigan</orgName><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Rocque, P A" sort="Rocque, P A" uniqKey="Rocque P" first="P A" last="Rocque">P A Rocque</name></author><author><name sortKey="Xu, D P" sort="Xu, D P" uniqKey="Xu D" first="D P" last="Xu">D P Xu</name></author><author><name sortKey="Meyer, E B" sort="Meyer, E B" uniqKey="Meyer E" first="E B" last="Meyer">E B Meyer</name></author><author><name sortKey="Charamella, L J" sort="Charamella, L J" uniqKey="Charamella L" first="L J" last="Charamella">L J Charamella</name></author><author><name sortKey="Dimitrov, N V" sort="Dimitrov, N V" uniqKey="Dimitrov N" first="N V" last="Dimitrov">N V Dimitrov</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:7750794</idno><idno type="pmid">7750794</idno><idno type="doi">10.1016/0891-5849(94)00188-p</idno><idno type="wicri:Area/Main/Corpus">001225</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001225</idno><idno type="wicri:Area/Main/Curation">001225</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001225</idno><idno type="wicri:Area/Main/Exploration">001225</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ascorbic acid and cell survival of adriamycin resistant and sensitive MCF-7 breast tumor cells.</title><author><name sortKey="Wells, W W" sort="Wells, W W" uniqKey="Wells W" first="W W" last="Wells">W W Wells</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, Michigan State University, East Lansing 48824, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry, Michigan State University, East Lansing 48824</wicri:regionArea><orgName type="university">Université d'État du Michigan</orgName><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Rocque, P A" sort="Rocque, P A" uniqKey="Rocque P" first="P A" last="Rocque">P A Rocque</name></author><author><name sortKey="Xu, D P" sort="Xu, D P" uniqKey="Xu D" first="D P" last="Xu">D P Xu</name></author><author><name sortKey="Meyer, E B" sort="Meyer, E B" uniqKey="Meyer E" first="E B" last="Meyer">E B Meyer</name></author><author><name sortKey="Charamella, L J" sort="Charamella, L J" uniqKey="Charamella L" first="L J" last="Charamella">L J Charamella</name></author><author><name sortKey="Dimitrov, N V" sort="Dimitrov, N V" uniqKey="Dimitrov N" first="N V" last="Dimitrov">N V Dimitrov</name></author></analytic><series><title level="j">Free radical biology & medicine</title><idno type="ISSN">0891-5849</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Ascorbic Acid (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Breast Neoplasms (MeSH)</term><term>Cell Survival (MeSH)</term><term>Cells, Cultured (drug effects)</term><term>Dose-Response Relationship, Drug (MeSH)</term><term>Doxorubicin (pharmacology)</term><term>Glutaredoxins (MeSH)</term><term>Humans (MeSH)</term><term>Oxidoreductases (MeSH)</term><term>Proteins (MeSH)</term><term>Rats (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide ascorbique (MeSH)</term><term>Animaux (MeSH)</term><term>Cellules cultivées (effets des médicaments et des substances chimiques)</term><term>Doxorubicine (pharmacologie)</term><term>Glutarédoxines (MeSH)</term><term>Humains (MeSH)</term><term>Oxidoreductases (MeSH)</term><term>Protéines (MeSH)</term><term>Rats (MeSH)</term><term>Relation dose-effet des médicaments (MeSH)</term><term>Survie cellulaire (MeSH)</term><term>Technique de Western (MeSH)</term><term>Tumeurs du sein (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Doxorubicin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Ascorbic Acid</term><term>Glutaredoxins</term><term>Oxidoreductases</term><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cells, Cultured</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Cellules cultivées</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Doxorubicine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Breast Neoplasms</term><term>Cell Survival</term><term>Dose-Response Relationship, Drug</term><term>Humans</term><term>Rats</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acide ascorbique</term><term>Animaux</term><term>Glutarédoxines</term><term>Humains</term><term>Oxidoreductases</term><term>Protéines</term><term>Rats</term><term>Relation dose-effet des médicaments</term><term>Survie cellulaire</term><term>Technique de Western</term><term>Tumeurs du sein</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ability of human cells to regenerate ascorbic acid from dehydroascorbate is partially dependent on the glutathione redox status of the cell and the relative activity of dehydroascorbate reductases. Mammalian dehydroascorbate reductase activity is associated with two proteins known as thioltransferase (glutaredoxin) and protein disulfide isomerase. We compared the specific activity of thioltransferase, protein disulfide isomerase, and other GSH-related enzymes in Adriamycin-resistant human breast tumor cells, MCF-7 ADRR, and Adriamycin-sensitive, MCF-7 WT, tumor cells. MCF-7 ADRR cells had higher activities of glutathione peroxidase (34.7 fold), nonseleno-glutathione peroxidase (glutathione S-transferases; 5.3 fold), thioredoxin (2.3 fold), and thioltransferase (4.0 fold) compared with the WT Adriamycin-sensitive cell line. Thioltransferase was detected in Western blots in extracts of ADRR MCF-7 cells but not in WT MCF-7 cells. alpha-Tocopherol in the membrane and cytosolic fractions was 2.8 and 3.0 fold higher, respectively, in Adriamycin-resistant compared with Adriamycin-sensitive cells. Supplementation of MCF-7 cells with L-ascorbic acid 2-phosphate (2 and 10 mM) had no effect on WT cell viability after 5 days incubation with up to 0.33 microM Adriamycin. In contrast, supplementation of ADRR MCF-7 cells with L-ascorbic acid 2-phosphate resulted in enhanced resistance up to 3.4 microM Adriamycin over a 5-day incubation. Both lines of MCF-7 cells demonstrated the ability to utilize ascorbic acid as the 2-phosphate derivative. After 48 h incubation with 8.6 microM Adriamycin, the resistant cells maintained normal viability and ascorbate-dehydroascorbate levels, whereas drug-sensitive cells had significantly lower ascorbate with a higher percent dehydroascorbate and increased cell death as judged by cell protein levels (52% of controls).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7750794</PMID><DateCompleted><Year>1995</Year><Month>06</Month><Day>21</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0891-5849</ISSN><JournalIssue CitedMedium="Print"><Volume>18</Volume><Issue>4</Issue><PubDate><Year>1995</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Free radical biology & medicine</Title><ISOAbbreviation>Free Radic Biol Med</ISOAbbreviation></Journal><ArticleTitle>Ascorbic acid and cell survival of adriamycin resistant and sensitive MCF-7 breast tumor cells.</ArticleTitle><Pagination><MedlinePgn>699-708</MedlinePgn></Pagination><Abstract><AbstractText>The ability of human cells to regenerate ascorbic acid from dehydroascorbate is partially dependent on the glutathione redox status of the cell and the relative activity of dehydroascorbate reductases. Mammalian dehydroascorbate reductase activity is associated with two proteins known as thioltransferase (glutaredoxin) and protein disulfide isomerase. We compared the specific activity of thioltransferase, protein disulfide isomerase, and other GSH-related enzymes in Adriamycin-resistant human breast tumor cells, MCF-7 ADRR, and Adriamycin-sensitive, MCF-7 WT, tumor cells. MCF-7 ADRR cells had higher activities of glutathione peroxidase (34.7 fold), nonseleno-glutathione peroxidase (glutathione S-transferases; 5.3 fold), thioredoxin (2.3 fold), and thioltransferase (4.0 fold) compared with the WT Adriamycin-sensitive cell line. Thioltransferase was detected in Western blots in extracts of ADRR MCF-7 cells but not in WT MCF-7 cells. alpha-Tocopherol in the membrane and cytosolic fractions was 2.8 and 3.0 fold higher, respectively, in Adriamycin-resistant compared with Adriamycin-sensitive cells. Supplementation of MCF-7 cells with L-ascorbic acid 2-phosphate (2 and 10 mM) had no effect on WT cell viability after 5 days incubation with up to 0.33 microM Adriamycin. In contrast, supplementation of ADRR MCF-7 cells with L-ascorbic acid 2-phosphate resulted in enhanced resistance up to 3.4 microM Adriamycin over a 5-day incubation. Both lines of MCF-7 cells demonstrated the ability to utilize ascorbic acid as the 2-phosphate derivative. After 48 h incubation with 8.6 microM Adriamycin, the resistant cells maintained normal viability and ascorbate-dehydroascorbate levels, whereas drug-sensitive cells had significantly lower ascorbate with a higher percent dehydroascorbate and increased cell death as judged by cell protein levels (52% of controls).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wells</LastName><ForeName>W W</ForeName><Initials>WW</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, Michigan State University, East Lansing 48824, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rocque</LastName><ForeName>P A</ForeName><Initials>PA</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>D P</ForeName><Initials>DP</Initials></Author><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>E B</ForeName><Initials>EB</Initials></Author><Author ValidYN="Y"><LastName>Charamella</LastName><ForeName>L J</ForeName><Initials>LJ</Initials></Author><Author ValidYN="Y"><LastName>Dimitrov</LastName><ForeName>N V</ForeName><Initials>NV</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CA-51972</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>N01-CN-45167</GrantID><Acronym>CN</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Free Radic Biol Med</MedlineTA><NlmUniqueID>8709159</NlmUniqueID><ISSNLinking>0891-5849</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C516010">Glrx2 protein, rat</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>80168379AG</RegistryNumber><NameOfSubstance UI="D004317">Doxorubicin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>PQ6CK8PD0R</RegistryNumber><NameOfSubstance UI="D001205">Ascorbic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001205" MajorTopicYN="Y">Ascorbic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001943" MajorTopicYN="Y">Breast Neoplasms</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004317" MajorTopicYN="N">Doxorubicin</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="Y">Oxidoreductases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>4</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">7750794</ArticleId><ArticleId IdType="pii">089158499400188P</ArticleId><ArticleId IdType="doi">10.1016/0891-5849(94)00188-p</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region><settlement><li>East Lansing</li></settlement><orgName><li>Université d'État du Michigan</li></orgName></list><tree><noCountry><name sortKey="Charamella, L J" sort="Charamella, L J" uniqKey="Charamella L" first="L J" last="Charamella">L J Charamella</name><name sortKey="Dimitrov, N V" sort="Dimitrov, N V" uniqKey="Dimitrov N" first="N V" last="Dimitrov">N V Dimitrov</name><name sortKey="Meyer, E B" sort="Meyer, E B" uniqKey="Meyer E" first="E B" last="Meyer">E B Meyer</name><name sortKey="Rocque, P A" sort="Rocque, P A" uniqKey="Rocque P" first="P A" last="Rocque">P A Rocque</name><name sortKey="Xu, D P" sort="Xu, D P" uniqKey="Xu D" first="D P" last="Xu">D P Xu</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Wells, W W" sort="Wells, W W" uniqKey="Wells W" first="W W" last="Wells">W W Wells</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GlutaredoxinV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001232 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001232 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GlutaredoxinV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:7750794
|texte= Ascorbic acid and cell survival of adriamycin resistant and sensitive MCF-7 breast tumor cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:7750794" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GlutaredoxinV1
| This area was generated with Dilib version V0.6.37. |  |